Sample Set Annotation Tool: Profiling of naïve and memory Tregs-GSE76598

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Changes in Treg function are difficult to quantify due to the lack of Treg-exclusive markers in humans and the complexity of functional experiments. We sorted naive and ...

Hypothesis

Experimental Design

7 adult healthy control samples were sorted into 4 subsets: naïve (CD4+CD25hiCD45RA+) and memory (CD4+CD25hiCD45RA-) Tregs, and naïve (CD4+CD25-CD45RA+) and memory ...

Experimental Variables

Controls

Methods

Additional Information

Pesenacker AM, Wang AY, Singh A, Gillies J et al. A Regulatory T-Cell Gene Signature Is a Specific and Sensitive Biomarker to Identify Children With New-Onset Type 1 ...

Expected Samples:

52 Samples Loaded: 52

Species

Human (Homo sapiens)

Sample Source

Regulatory T Cells

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	individual	treatment	cell subset	Grouping
GSM2028716	MLS010	donor 1	unstimulated	naiveTreg	Non_naiveTreg
GSM2028717	MLS011	donor 1	unstimulated	memoryTconv	Non_mTcov
GSM2028718	MLS014x	donor 1	stimulated	naiveTreg	Sti_naiveTreg
GSM2028719	MLS015	donor 7	stimulated	memoryTconv	Sti_mTconv
GSM2028720	MLS016	donor 1	stimulated	naiveTconv	Sti_naiveTconv

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File
sampleset4000036_sampleannotations (1).csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Individual	Treatment	Cell subset	Grouping
GSM2028716	MLS010	donor 1	unstimulated	naiveTreg	Non_naiveTreg
GSM2028717	MLS011	donor 1	unstimulated	memoryTconv	Non_mTcov
GSM2028718	MLS014x	donor 1	stimulated	naiveTreg	Sti_naiveTreg
GSM2028719	MLS015	donor 7	stimulated	memoryTconv	Sti_mTconv
GSM2028720	MLS016	donor 1	stimulated	naiveTconv	Sti_naiveTconv
GSM2028721	MLS025	donor 1	unstimulated	memoryTreg	Non_mTreg
GSM2028722	MLS026	donor 2	unstimulated	naiveTreg	Non_naiveTreg
GSM2028723	MLS027	donor 2	unstimulated	memoryTconv	Non_mTcov
GSM2028724	MLS028	donor 1	unstimulated	naiveTconv	Non_naiveTconv
GSM2028725	MLS029	donor 1	stimulated	memoryTreg	Sti_mTreg
GSM2028726	MLS030	donor 2	stimulated	naiveTreg	Sti_naiveTreg
GSM2028727	MLS031	donor 1	stimulated	memoryTconv	Sti_mTconv
GSM2028728	MLS032	donor 2	stimulated	naiveTconv	Sti_naiveTconv
GSM2028729	MLS033	donor 2	unstimulated	memoryTreg	Non_mTreg
GSM2028730	MLS034	donor 3	unstimulated	naiveTreg	Non_naiveTreg
GSM2028731	MLS035	donor 3	unstimulated	memoryTconv	Non_mTcov
GSM2028732	MLS036	donor 2	unstimulated	naiveTconv	Non_naiveTconv
GSM2028733	MLS037	donor 2	stimulated	memoryTreg	Sti_mTreg
GSM2028734	MLS038	donor 3	stimulated	naiveTreg	Sti_naiveTreg
GSM2028735	MLS039	donor 2	stimulated	memoryTconv	Sti_mTconv
GSM2028736	MLS040	donor 3	stimulated	naiveTconv	Sti_naiveTconv
GSM2028737	MLS041	donor 3	unstimulated	memoryTreg	Non_mTreg
GSM2028738	MLS042	donor 4	unstimulated	naiveTreg	Non_naiveTreg
GSM2028739	MLS044	donor 3	unstimulated	naiveTconv	Non_naiveTconv
GSM2028740	MLS045	donor 3	stimulated	memoryTreg	Sti_mTreg
GSM2028741	MLS046	donor 4	stimulated	naiveTreg	Sti_naiveTreg
GSM2028742	MLS047	donor 3	stimulated	memoryTconv	Sti_mTconv
GSM2028743	MLS048	donor 4	stimulated	naiveTconv	Sti_naiveTconv
GSM2028744	MLS065	donor 4	unstimulated	memoryTreg	Non_mTreg
GSM2028745	MLS066x	donor 5	unstimulated	naiveTreg	Non_naiveTreg
GSM2028746	MLS067x	donor 4	unstimulated	memoryTconv	Non_mTcov
GSM2028747	MLS068	donor 4	unstimulated	naiveTconv	Non_naiveTconv
GSM2028748	MLS069x	donor 4	stimulated	memoryTreg	Sti_mTreg
GSM2028749	MLS070	donor 5	stimulated	naiveTreg	Sti_naiveTreg
GSM2028750	MLS071	donor 4	stimulated	memoryTconv	Sti_mTconv
GSM2028751	MLS072	donor 5	stimulated	naiveTconv	Sti_naiveTconv
GSM2028752	MLS073x	donor 5	unstimulated	memoryTreg	Non_mTreg
GSM2028753	MLS074x	donor 6	unstimulated	naiveTreg	Non_naiveTreg
GSM2028754	MLS075	donor 5	unstimulated	memoryTconv	Non_mTcov
GSM2028755	MLS076	donor 5	unstimulated	naiveTconv	Non_naiveTconv
GSM2028756	MLS077x	donor 5	stimulated	memoryTreg	Sti_mTreg
GSM2028757	MLS078	donor 6	stimulated	naiveTreg	Sti_naiveTreg
GSM2028758	MLS079	donor 5	stimulated	memoryTconv	Sti_mTconv
GSM2028759	MLS080	donor 6	stimulated	naiveTconv	Sti_naiveTconv
GSM2028760	MLS081x	donor 6	unstimulated	memoryTreg	Non_mTreg
GSM2028761	MLS082	donor 7	unstimulated	naiveTreg	Non_naiveTreg
GSM2028762	MLS083	donor 6	unstimulated	memoryTconv	Non_mTcov
GSM2028763	MLS084x	donor 6	unstimulated	naiveTconv	Non_naiveTconv
GSM2028764	MLS085	donor 6	stimulated	memoryTreg	Sti_mTreg
GSM2028765	MLS086	donor 7	stimulated	naiveTreg	Sti_naiveTreg
GSM2028766	MLS087	donor 6	stimulated	memoryTconv	Sti_mTconv
GSM2028767	MLS088x	donor 7	stimulated	naiveTconv	Sti_naiveTconv

Group Sets View in Gene Expression Browser

Name
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Culture conditions	Default	View
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Module Analysis

Group Set
All Samples
Culture conditions

SIDRA