Sample Set Annotation Tool: Expression data of A2EN cells during early stage of Chlamydia trachomatis infection-GSE54336

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Used for fold change calculations

Hypothesis

Experimental Design

A2EN cells were mock infected, or infected with a Chlamydia mutant or its complemented counterpart for 4 hour post infection.

Experimental Variables

Controls

Methods

The data were analyzed with Partek Genomic Suite 6.6 using Affymetrix default analysis settings, quantile normalization and RMA background correction.

Additional Information

This datasets was uploaded for the purpose of the fold change calculations.

Expected Samples:

6 Samples Loaded: 6

Species

Human (Homo sapiens)

Sample Source

Disease

Chlamydia trachomatis infection

Treatment Protocol

A2EN cells were infected with different strains of Chlamydia trachomatis at an MOI of 10 by spinning at 3000 rpm for 30 min at 10 degree and shift to 37 degree incubator for 4 hours.

Growth Protocol

Human endocervical epithelial A2EN cells were maintained in keratinocyte-SFM medium supplemented with 10 % FBS, 0.5?ng/mL human recombinant epidermal growth factor and 50??g/mL bovine pituitary extract.

Extraction Protocol

Samples were collected using QIAGEN RNeasy Plus Mini Kit as described by the manufacturer.

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	time point	cell line	infected with
GSM1313408	A2EN cells_Chlamydia G1TEPP_4h_rep1	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1TEPP; complemented strain
GSM1313409	A2EN cells_Chlamydia G1TEPP_4h_rep2	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1TEPP; complemented strain
GSM1313410	A2EN cells_Chlamydia G1V_4h_rep1	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1V mutant
GSM1313411	A2EN cells_Chlamydia G1V_4h_rep2	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1V mutant
GSM1313412	A2EN cells_Mock infected_repl1	Human endocervical epithelial cells	4 hr post infection	A2EN	none (mock)

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Sample ID	!Sample Title	Cell type	Time point	Cell line	Infected with
GSM1313408	A2EN cells_Chlamydia G1TEPP_4h_rep1	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1TEPP; complemented strain
GSM1313409	A2EN cells_Chlamydia G1TEPP_4h_rep2	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1TEPP; complemented strain
GSM1313410	A2EN cells_Chlamydia G1V_4h_rep1	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1V mutant
GSM1313411	A2EN cells_Chlamydia G1V_4h_rep2	Human endocervical epithelial cells	4 hr post infection	A2EN	Chlamydia G1V mutant
GSM1313412	A2EN cells_Mock infected_repl1	Human endocervical epithelial cells	4 hr post infection	A2EN	none (mock)
GSM1313413	A2EN cells_Mock infected_repl2	Human endocervical epithelial cells	4 hr post infection	A2EN	none (mock)

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