Sample Set Annotation Tool: Peripheral blood cells expression data form 7 patients with severe pdm(H1N1) influensa and 7 gender and age matched healthy controls-GSE27131

Study Design
Assay
Sample Info
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Info
Samples
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Analysis

Purpose

Using PAXgene tubes, peripheral blood samples were collected from seven patients >18 years with documented pdm(H1N1) influenza, bilateral chest infiltrates, and in need of ventilation support. Significant co-morbidity was exclusion criterion. Expression profiles were compared with 7 age matched controls. Using a false discovery rate < 5% and an absolute fold change > 2, 370 genes were differentially expressed in case and controls.A second sample was collected after ca 6 days in the 7 patients and temporal changes in expression profiles investigated. Over expressed putative mediators of inflammatory lung dammage were measured on the protein level.

Hypothesis

Experimental Design

14 patient samples (7 paired samples) and 7 control samples were performed.

Experimental Variables

Controls

Methods

Raw data were processed using Partek Genomics Suite v. 6.5 to generate RMA normalised intensities. Differential expression was assessed using One-way or Two-way ANOVA in the Partek software dependent of if the samples were paired or not.

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27131

Expected Samples:

21 Samples Loaded: 21

Species

Human (Homo sapiens)

Sample Source

Peripheral blood

Disease

H1N1

Treatment Protocol

Blood samples were collected with PAXgene Blood RNA tubes

Growth Protocol

Peripheral blood cells

Extraction Protocol

RNA extraction was performed with PAXgene Blood RNA kit according to manufactors instructions, and RNA quality assesed with Bioanalyzer

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Age	Gender	!Sample_title	tissue
GSM670449	54	male	blood_day0_patient1	blood
GSM670450	54	male	blood_day6_patient1	blood
GSM670451	38	male	blood_day0_patient2	blood
GSM670452	38	male	blood_day6_patient2	blood
GSM670453	59	male	blood_day0_patient3	blood

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File

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Sample ID	Age	Gender	!Sample Title	Tissue	Control	Patient
GSM670449	54	male	blood_day0_patient1	blood		H1N1
GSM670450	54	male	blood_day6_patient1	blood		H1N1
GSM670451	38	male	blood_day0_patient2	blood		H1N1
GSM670452	38	male	blood_day6_patient2	blood		H1N1
GSM670453	59	male	blood_day0_patient3	blood		H1N1
GSM670454	59	male	blood_day6_patient3	blood		H1N1
GSM670455	28	female	blood_day0_patient4	blood		H1N1
GSM670456	28	female	blood_day6_patient4	blood		H1N1
GSM670457	38	male	blood_day0_patient5	blood		H1N1
GSM670458	38	male	blood_day6_patient5	blood		H1N1
GSM670459	46	male	blood_day0_patient6	blood		H1N1
GSM670460	46	male	blood_day6_patient6	blood		H1N1
GSM670461	25	male	blood_day0_patient7	blood		H1N1
GSM670462	25	male	blood_day6_patient7	blood		H1N1
GSM670463	41	male	Blood_Control 1	blood	Control
GSM670464	48	male	Blood_Control 2	blood	Control
GSM670465	38	female	Blood_Control 3	blood	Control
GSM670466	26	male	Blood_Control 4	blood	Control
GSM670467	41	male	Blood_Control 5	blood	Control
GSM670468	40	female	Blood_Control 6	blood	Control
GSM670469	35	female	Blood_Control 7	blood	Control

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Module Analysis

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