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Clinical

CHANGES IN THE HUMAN TRANSCRIPTOME UPON VITAMIN D SUPPLEMENTATION-GSE94138

Purpose
Subjects of a randomized controlled trial (RCT) received either vitamin D3 (n = 47) in a weekly dose of 20,000 IU or placebo (n = 47) for a period of three to five years
Hypothesis

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Experimental Design

Blood samples for preparation of RNA were drawn from the subjects and mRNA gene expression in blood was determined using microarray analyses.

Experimental Variables

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Controls

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Methods
The data was preprocessed by Bioconductor lumi package (version 2.26.4). It was log2 transformed and quantile normalized.
Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE94138

Microarray
Illumina HumanHT-12 v4
15 Samples Loaded: 15
Human (Homo sapiens)
Whole Blood
Vitamin D
standard as recommended by Illumina
Sample Set Spreadsheet
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Samples Preview
Sample ID Albumin Weight !Sample_title tissue Sex
GSM2470912 47 89.8 vitD:20215292 blood male
GSM2470914 45.5 73.1 vitD:20215297 blood male
GSM2470915 44.7 96.1 vitD:20215299 blood male
GSM2470917 45.2 95.3 vitD:20215305 blood male
GSM2470918 44.9 97.5 vitD:20215306 blood male
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File
Log2 Transformed
Raw Signal
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Sample ID Albumin Weight !Sample Title Tissue Sex Treatment Bmi Calcium Dnaassay Phosphate Sample Month Date SampleID Smoke Height Pth Dbp
GSM2470912
47
89.8
vitD:20215292
blood
male
vitamin D
28.66
2.32
271
0.84
3
20215292
0
177
4.1
3.206
GSM2470914
45.5
73.1
vitD:20215297
blood
male
Placebo
23.07
2.3
278
0.95
3
20215297
1
178
7.2
2.531
GSM2470915
44.7
96.1
vitD:20215299
blood
male
vitamin D
31.74
2.23
274
0.98
3
20215299
0
174
3.8
3.176
GSM2470917
45.2
95.3
vitD:20215305
blood
male
Placebo
30.08
2.25
280
0.81
3
20215305
0
178
6.9
2.869
GSM2470918
44.9
97.5
vitD:20215306
blood
male
Placebo
28.8
2.32
282
0.97
3
20215306
0
184
5.2
2.692
GSM2470919
45.1
72.8
vitD:20215307
blood
female
vitamin D
27.4
2.38
284
1.14
3
20215307
0
163
6.2
3.116
GSM2470952
45.3
102.4
vitD:20215544
blood
male
vitamin D
33.82
2.49
517
0.91
3
20215544
0
174
3.7
3.038
GSM2470954
43.2
103.3
vitD:20215546
blood
male
vitamin D
35.33
2.23
510
0.98
3
20215546
0
171
6.2
2.979
GSM2470955
42.9
89.5
vitD:20215548
blood
male
Placebo
29.22
2.26
507
1.05
3
20215548
0
175
4.6
3.337
GSM2470956
43.5
90.3
vitD:20215549
blood
male
vitamin D
27.56
2.27
508
1.26
3
20215549
0
181
5.6
2.762
GSM2470957
48.2
92.7
vitD:20215551
blood
male
vitamin D
28.61
2.48
513
0.84
3
20215551
0
180
6.9
2.487
GSM2470958
45.8
76.1
vitD:20215552
blood
male
Placebo
28.64
2.34
515
1.21
3
20215552
0
163
2.7
2.807
GSM2470959
46.4
77.6
vitD:20215553
blood
male
vitamin D
27.82
2.41
512
1.01
3
20215553
0
167
5.9
2.199
GSM2470960
44.8
93.5
vitD:20215555
blood
male
Placebo
30.88
2.25
516
1.26
3
20215555
0
174
6.3
3.036
GSM2470961
40.3
95.7
vitD:20215557
blood
male
vitamin D
31.25
2.16
520
0.95
3
20215557
1
175
4.4
2.886

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