Sample Set Annotation Tool: Expression data from Treg subsets sorted from healthy human peripheral blood-GSE64176

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

All the sorted Treg cells are gated on CD4+CD25+CD127(low), followed by gating on various subsets using markers indicated in the sample names.All the sorted Teff cells are gated on CD4+CD25-CD127(hi), and further sorted for CD45RO- and CD45RO+ phenotype.

Hypothesis

Experimental Design

Each Treg and Teff cell subsets were sorted from three different donors. We sought to identify gene expression signature characteristic to specific Treg subsets

Experimental Variables

Controls

Methods

Additional Information

Hua J, Davis SP, Hill JA, Yamagata T. Diverse Gene Expression in Human Regulatory T Cell Subsets Uncovers Connection between Regulatory T Cell Genes and Suppressive Function. J Immunol 2015 Oct 15;195(8):3642-53. PMID: 26371251

Expected Samples:

12 Samples Loaded: 12

Species

Human (Homo sapiens)

Sample Source

Regulatory T Cells

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	sample type	Day
GSM1565927	Teff.CD45RO-_d1.rep1	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565928	Teff.CD45RO-_d1.rep2	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565929	Teff.CD45RO-_d1.rep3	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565930	Teff.CD45RO+_d1.rep1	Teff CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565931	Teff.CD45RO+_d1.rep2	Teff CD45RO+_d1	Sorted ex vivo Treg subset	Day1

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File
sampleset4000037_sampleannotations.csv

Samples Viewer / Editor

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Sample ID	!Sample Title	Cell type	Sample type	Day
GSM1565927	Teff.CD45RO-_d1.rep1	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565928	Teff.CD45RO-_d1.rep2	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565929	Teff.CD45RO-_d1.rep3	Teff CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565930	Teff.CD45RO+_d1.rep1	Teff CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565931	Teff.CD45RO+_d1.rep2	Teff CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565932	Teff.CD45RO+_d1.rep3	Teff CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565933	Treg.CD45RO-_d1.rep1	Treg CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565934	Treg.CD45RO-_d1.rep2	Treg CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565935	Treg.CD45RO-_d1.rep3	Treg CD45RO-_d1	Sorted ex vivo Treg subset	Day1
GSM1565936	Treg.CD45RO+_d1.rep1	Treg CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565937	Treg.CD45RO+_d1.rep2	Treg CD45RO+_d1	Sorted ex vivo Treg subset	Day1
GSM1565938	Treg.CD45RO+_d1.rep3	Treg CD45RO+_d1	Sorted ex vivo Treg subset	Day1

Group Sets View in Gene Expression Browser

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Module Analysis

Group Set
All Samples
Cell type

SIDRA